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Partial characterization of α-mannosidase from Yarrowia lipolytica

✍ Scribed by Rosario Vega; Dr. Angel Domínguez

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
499 KB
Volume
28
Category
Article
ISSN
0233-111X

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✦ Synopsis

An exo-a-mannosidase (E.C. 3.2.1.24) was characterized in the yeast form of Yarrowia lipolytica. The enzyme located in a crude particulate fraction of the cell extract is under catabolite repression, has an optimum pH of 6.0, a Km of 0.27 m~ with p-nitrophenyl-lx-o-mannopyranoside and is partially inhibited by D-mannose.

The enzyme is not affected by ethylenediaminotetraacetic acid, several cations (only Zn' + increased 'its activity in a 25%) or sulphydryl reagents and can be partially solubilized by treatment with digitonine.

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