Partial characterization of 5-fluoropyrimidine-resistant mutants of Neurospora crassa
β Scribed by Buxton, Frank P. ;Radford, Alan
- Publisher
- Springer
- Year
- 1982
- Tongue
- English
- Weight
- 358 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0026-8925
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Wild type Neurospora crassa was shown to be more resistant to 5-fluoro-uracil, 5-fluoro-uridine and 5-fluoro-2'-deoxyuridine in the presence of ammonia than in its absence. This differential resistance may in part be accounted for by the observation that both uracil and uridine uptake in germinating
Non-saponifiable cell extracts of wild type and sterol mutants of N. crassa were analysed by means of gas-liquid chromatography. The wild-type contained ergosterol and episertol in a 10:1 ratio. None of the mutants was able to synthesize ergosterol. Three of the mutants carry single recessive gene m