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PARENCHYMAL CELLS FROM ADULT RAT LIVER IN NONPROLIFERATING MONOLAYER CULTURE: I. Functional Studies

✍ Scribed by Montgomery Bissell, D.

Book ID
111652145
Publisher
Rockefeller University Press.
Year
1973
Tongue
English
Weight
992 KB
Volume
59
Category
Article
ISSN
0021-9525

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Establishment of monolayer cell-cultures from adult normal rat liver is described using cells isolated by a collagenase-hyaluronidase perfusion technique. The cells plated originally were polygonal in shape and contained a large number of mitochondria. However, cells with less granules were also obs

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## Abstract Parenchymal cells were isolated from adult rat liver with an enzyme perfusion technique. The single‐cell suspension, representing 40–50% of the liver's hepatocytes was suspended in medium and maintained in primary culture for up to four days. The cells were found to carry out glycogen s