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Parathyroid hormone stimulation of mitosis in rat thymic lymphocytes is independent of cyclic AMP

โœ Scribed by M.J. Atkinson; R.-D. Hesch; C. Cade; M. Wadwah; A.D. Perris


Book ID
112118262
Publisher
American Society for Bone and Mineral Research
Year
2009
Tongue
English
Weight
597 KB
Volume
2
Category
Article
ISSN
0884-0431

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## Abstract Purified parathyroid hormone (PTH) strongly stimulates the initiation of deoxyribonucleic acid (DNA) synthesis and thereby raises the flow of cells into mitosis in rat thymic lymphocyte populations maintained __in vitro__. These actions of PTH are potentiated by caffeine and inhibited b

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## Cell culture The opossum kidney (OK) cells, kindly provided by Dr. J. Handler, National Institutes of Health, Bethesda, Maryland, were seeded at a density of 1.2 x 104/cm2 in DMEM, supplemented with 10% FCS, containing 100 pg/ml of streptomycin and 100 IU/ml of penicillin. Medium was replaced o