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Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser-induced fluorescence detection

✍ Scribed by Irena Nikcevic; Aigars Piruska; Kenneth R. Wehmeyer; Carl J. Seliskar; Patrick A. Limbach; William R. Heineman


Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
285 KB
Volume
31
Category
Article
ISSN
0173-0835

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## Abstract Carbonyl‐modified proteins are markers of oxidative damage. Here, we report a new method for detecting and quantifying carbonylated proteins by capillary sieving electrophoresis (CSE) with LIF detection (CSE‐LIF). Alexa 488 hydrazide is used for the specific labeling of carbonyls while