Paper Chromatography of sugar phosphates and three-carbon phosphates extension and modification of the agarwal procedure

✍ Scribed by Mary H. Subers; Abner I. Schepartz; Robert P. Koob

• Book ID: 104147152
• Publisher: Elsevier Science
• Year: 1966
• Tongue: English
• Weight: 574 KB
• Volume: 23
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(01)98693-5

✦ Synopsis

The results given in Table :I1 show that whenever triethanolamine : was ;incorporated in a formulation, mono-and di-ethanolamine 'were present !as impurities. : When the chromatogram'is'sprayed with ninhydrin the red zone (@ 0.60). due to a carboxylic acidoverlays and conceals the zone,of triethanolamine (&P 0;60) when both:the acid and amine are present in a mixture, However, if triethanolamine is'presentsit: can be confirmed by the distinct purple hue of tlie red zone when sprayed with alizarin solution. Sometimes the other components of 'a formulation,cause the acid constituent to appear as a red streak from Xp CY 0.3 to & w 0.6 but this does not prevent identification of the aikanolamine constituents.

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