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Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of 13C-labelled C4 to C7 sugar phosphates

✍ Scribed by John K. MacLeod; Ian L. Flanigan; John F. Williams; J. Grant Collins

Publisher: John Wiley and Sons
Year: 2001
Tongue: English
Weight: 160 KB
Volume: 36
Category: Article
ISSN: 1076-5174
DOI: 10.1002/jms.147

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✦ Synopsis

Abstract

The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime‐ and ethoxime‐trimethylsilyl (TMS) derivatives of C~4~ to C~7~ sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled ^13^C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon–carbon bond cleavages of the straight‐chain derivatives. The results confirm that GC/MS of the alkoxime‐TMS derivatives is a viable method for measuring ^13^C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with ^13^CO~2~. Copyright © 2001 John Wiley & Sons, Ltd.

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