## Abstract We show how electron microscopy can be used to answer several critical issues in neurodegenerative disorders that course with the formation of aberrant filamentous structures. Thus, electron microscopy is a useful technique to study in vitro assembly of pathogenic proteins, to map the r
Paired Helical Filaments in Electron Microscopy of Alzheimer's Disease
β Scribed by KIDD, M.
- Book ID
- 109626033
- Publisher
- Nature Publishing Group
- Year
- 1963
- Tongue
- English
- Weight
- 384 KB
- Volume
- 197
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/197192b0
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Results of this comparative study indicated that electron microscopy of supra-ultrathin sectipns provided the best resolution; revealing the subunit structures, globules (32 A +4), and longitudinal bars (47 A f6) of paired helical filaments (PHF). A three-dimensional model of PHF substructure is the
## Abstract Paired helical filaments (PHFs) are abnormal twisted filaments composed of hyperphosphorylated tau protein. They are found in Alzheimer's disease and other neurodegenerative disorders designated as tauopathies. They are a major component of intracellular inclusions known as neurofibrill
A competitive enzyme-linked immunosorbent assay with high sensitivity has been developed for measuring ubiquitin reactivity of paired helical filaments (PHF). Using the assay, ubiquitin immunoreactivity was estimated in the cerebrospinal fluid (CSF) of 44 patients who had been clinically diagnosed a