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Characterization of Alzheimer paired helical filaments by electron microscopy

✍ Scribed by Félix Hernández; Tobias Engel; Alberto Gómez-Ramos; Mar Pérez; Jesús Avila

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
112 KB
Volume
67
Category
Article
ISSN
1059-910X

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✦ Synopsis

Abstract

We show how electron microscopy can be used to answer several critical issues in neurodegenerative disorders that course with the formation of aberrant filamentous structures. Thus, electron microscopy is a useful technique to study in vitro assembly of pathogenic proteins, to map the regions involved in filament formation, as well as to detect by immunoelectron microscopy which proteins bind to the filaments. Furthermore, electron microscopy is the main technique used to discover if an animal model develops fibrillar pathology and if those filaments are similar to those found in human patients. This review focuses on Alzheimer's disease and related tauopathies, although similar studies have been done with other neurodegenerative disorders as, for example, Huntington's disease. Microsc. Res. Tech. 67:121–125, 2005. © 2005 Wiley‐Liss, Inc.

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