Packaging double-helical DNA into viral capsids

✍ Scribed by Jaclyn C. LaMarque; Thuc-vy L. Le; Stephen C. Harvey

• Book ID: 101712829
• Publisher: Wiley (John Wiley & Sons)
• Year: 2004
• Tongue: English
• Weight: 226 KB
• Volume: 73
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.10529

✦ Synopsis

Abstract

DNA packaging in bacteriophage P4 has been examined using a molecular mechanics model with a reduced representation containing one pseudoatom per turn of the double helix. The model is a discretized version of an elastic continuum model. The DNA is inserted piecewise into the model capsid, with the structure being reoptimized after each piece is inserted. Various optimization protocols were investigated, and it was found that molecular dynamics at a very low temperature (0.3 K) produces the optimal packaged structure. This structure is a concentric spool, rather than the coaxial spool that has been commonly accepted for so many years. This geometry, which was originally suggested by Hall and Schellman in 1982 (Biopolymers Vol. 21, pp. 2011–2031), produces a lower overall elastic energy than coaxial spooling. © 2003 Wiley Periodicals, Inc. Biopolymers 73: 348–355, 2004