Building of RNA and DNA double helices into electron density

✍ Scribed by Pavelcik, Frantisek ;Schneider, Bohdan

• Book ID: 104478396
• Publisher: International Union of Crystallography
• Year: 2008
• Tongue: English
• Weight: 654 KB
• Volume: 64
• Category: Article
• ISSN: 0907-4449
• DOI: 10.1107/s0907444908007075

✦ Synopsis

A method has been developed that automatically fits doublehelical regions into the electron density of nucleic acid structures. Rigid fragments consisting of two Watson-Crick base pairs and three pairs of phosphate groups in the A-type or B-type conformation are positioned into the electron density by phased rotation and translation functions. The position and orientation of the localized double-helical fragments are determined by phased refinement. The method has been tested by building double-helical regions of nine RNA structures of variable crystallographic resolution and polynucleotide length and is available for free use.