𝔖 Bobbio Scriptorium
✦   LIBER   ✦

p75NTR induces apoptosis in medulloblastoma cells

✍ Scribed by Jan Küchler; Wolfgang Hartmann; Anke Waha; Arend Koch; Elmar Endl; Peter Wurst; Dagmar Kindler; Thomas Mikeska; Andreas Waha; Cynthia G. Goodyer; Reinhard Büttner; Karl Schilling; Torsten Pietsch

Publisher
John Wiley and Sons
Year
2010
Tongue
French
Weight
366 KB
Volume
128
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The classic medulloblastoma (CMB) and the desmoplastic medulloblastoma (DMB) subtypes represent the major medulloblastoma variants. In contrast to CMB, DMB display high levels of the low‐affinity nerve growth factor receptor p75^NTR^. Given the reports of a better clinical course of DMB, we hypothesized that p75^NTR^ might act as a tumor suppressor in medulloblastomas. In a large set of medulloblastomas, p75^NTR^ was screened for mutations, and its mRNA expression and the DNA methylation status of its 5′‐region were assessed. p75^NTR^ immunostainings were performed in wild‐type murine cerebella and medulloblastomas arising in patched heterozygous mice, and murine cerebellar granule cell precursors (GCP) were analyzed in vitro. Medulloblastoma cells engineered to express p75^NTR^ were characterized flow cytometrically and morphologically. One CMB displayed a mutation of the p75^NTR^ coding sequence. p75^NTR^ mRNA levels clearly delineated DMB and CMB; however, CpG island hypermethylation was excluded as the cause of low p75^NTR^ expression in CMB. Sonic Hedgehog‐treated GCP showed elevated p75^NTR^ expression, and strong expression of p75^NTR^ was detected in the external granule cell layer of wild‐type mice and in murine ptc^±^ medulloblastomas. CMB cells overexpressing p75^NTR^ displayed a significant increase in apoptosis. In summary, our data link activated Hedgehog signaling in DMB with p75^NTR^ expression and characterize p75^NTR^ as a biologically relevant inductor of apoptosis in MB.

📜 SIMILAR VOLUMES

The p75NTR tumor suppressor induces casp
The p75NTR tumor suppressor induces caspase-mediated apoptosis in bladder tumor cells
✍ Arshia Tabassum; Fatima Khwaja; Daniel Djakiew 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 French ⚖ 237 KB

## Abstract p75^NTR^ was identified as a tumor and metastasis suppressor that functions in part __via__ induction of apoptosis in tumor cells. To examine p75^NTR^‐dependent apoptosis in tumor cells, we demonstrated that a dose‐dependent increase in p75^NTR^ expression was associated with a concomit

NGF induces apoptosis in a human neurobl
NGF induces apoptosis in a human neuroblastoma cell line expressing the neurotrophin receptor p75NTR
✍ P. Kuner; C. Hertel 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 162 KB 👁 2 views

Nerve growth factor (NGF) has been demonstrated to support survival and differentiation of neuronal cells. Recently, a role of NGF in neuronal apoptosis has been suggested. NGF binds to tropomyosin receptor kinase A (TrkA) and to 75-kDa NGF receptor (p75 NTR ). TrkA is responsible for differentiatio

Roles of glial p75NTR in axonal regenera
Roles of glial p75NTR in axonal regeneration
✍ Xin-Fu Zhou; Hong-Yun Li 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

## Abstract The neurotrophin receptor p75 (p75NTR) is expressed by both neurons and glia. Nerve injury triggers up‐regulation of p75NTR in Schwann cells (SC) but not in central glia. In contrast to neuronal p75NTR, which mediates negative signals from myelin‐associated proteins resulting in neurite

Thrombin induces apoptosis in human tumo
Thrombin induces apoptosis in human tumor cells
✍ Rasheed Ahmad; Laurent Knafo; Jingwu Xu; Sardar T.A.K. Sindhu; José Menezes; Ali 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 French ⚖ 335 KB 👁 2 views
Molecular characterization of the loss o
Molecular characterization of the loss of p75NTR expression in human prostate tumor cells
✍ Scott Krygier; Daniel Djakiew 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 181 KB

## Abstract The low‐affinity nerve growth factor receptor p75^NTR^ is a 75‐kDa glycoprotein that belongs to the tumor necrosis factor receptor superfamily and has been implicated in the induction of apoptosis in various tissues and cell lines. Immunohistochemistry on tissue sections from radical pr