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P V.12 - P V.12 Repair of benzo[a]pyrene diol epoxide-DNA adducts in the DHFR gene of a human embryonic kidney cell line

✍ Scribed by Laura J. Schild; Charles Allen Smith; Philip C. Hanawalt; William M. Baird


Book ID
114071635
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
136 KB
Volume
379
Category
Article
ISSN
0027-5107

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## Abstract Benzo[__a__]pyrene diol epoxide (BPDE) was reacted __in vitro__ with (2'‐deoxy)nucleotides and with calf thymus DNA. The modified DNA was enzymatically hydrolyzed to the 5'‐monophosphate nucleotides using deoxyribonuclease I (DNA‐ase I), nuclease P1 and snake venom phosphodiesterase (SV