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Ozone response in wild type and radiation-sensitive mutants of Saccharomyces cerevisiae

✍ Scribed by Dubeau, Hélène ;Chung, Young S.

Publisher
Springer
Year
1979
Tongue
English
Weight
495 KB
Volume
176
Category
Article
ISSN
0026-8925

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✦ Synopsis

The effect of ozone exposure on Saccharomyces cerevisiae was studied. Factors such as ozone concentration, treatment time, media, initial cell concentration and growth phase were shown to influence ozone response in this organism. Logarithmic phase cells were much more sensitive than stationary phase cells to the lethal effect of ozone. The radiation-sensitive mutants rad3, rad6, rad51 and rad52 of S. cerevisiae were exposed, in water, to 50 ppm of ozone for 30 min. On comparing their survival curves, the rad51 and the rad52 mutants showed a greater sensitivity to ozone exposure than the wild type.

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