Oxygen gradients in animal-cell bioreactors
β Scribed by J. Tramper
- Book ID
- 104922000
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 626 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0920-9069
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
In large-scale bioreactors gradients often occur as a result From the time derivative dC1/dt and Eq. ( 2), the variation of the gas concentration C o can be computed. Continuing in this way, the trajectory for Co,~,, required to keep C2
Scale-up of stirred tank bioreactors from 0.02 m 3 to 0.3 m 3 commercial plant is discussed for hybridoma suspension culture. Schemes for dissolved oxygen control with sparged air in serum containing media are described as well as mechanical breakage of foam in small and large bioreactors. Porous me
Gradients of glucose in time and space are shown in a 30 m 3 cultivation of Saccharomyces cerevisiae grown in minimal medium to a cell density of 20 g 1-1. The fed-batch concept was used with glucose as the limiting component which was fed continuously to the process. As the mean glucose concentrati
Both kLa and kL measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s-' in a mechanically agitated animal cell culture vessel of working volume l .5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the t