𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Oxygen demand and supply in cell culture

✍ Scribed by Robert J. Fleischaker; Anthony J. Sinskey

Publisher
Springer
Year
1981
Tongue
English
Weight
419 KB
Volume
12
Category
Article
ISSN
1432-0614

No coin nor oath required. For personal study only.

✦ Synopsis

The oxygen demand of animal cells in microcarrier culture can be determined using a dissolved oxygen electrode. Continuous monitoring of the oxygen demand by human diploid fibroblasts shows it to be a good indicator of cell number. Furthermore, the oxygen demand of animal cells in large cultures can be supplied using membrane oxygenation using silicone rubber tubing. This method avoids the problems encountered with sparging the culture.

πŸ“œ SIMILAR VOLUMES

Polarographic measurement of oxygen supp
Polarographic measurement of oxygen supply and demand during aerobic propagation of a bacterial culture
✍ Lemp, John Frederick πŸ“‚ Article πŸ“… 1960 πŸ› Wiley (John Wiley & Sons) βš– 632 KB

## Abstract The sampling method of polarographic analysis for dissolved oxygen in culture medium is unaffected by the presence of viable micro‐organisms. This method allows measurement of peak oxygen demand, critical oxygen concentration, and oxygen supply during actual mass propagation of an aerob

Oxygen transfer in animal cell culture m
Oxygen transfer in animal cell culture medium
✍ M. Lavery; A. W. Nienow πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 615 KB

Both kLa and kL measurements were carried out by an unsteady state technique at impeller speeds ranging from 1.6 to 5.8 s-' in a mechanically agitated animal cell culture vessel of working volume l .5 L. Checks were made that the time constant of the oxygen electrode was negligible compared to the t