Oxygen and carbon dioxide exchanges in the turtle's liver

✍ Scribed by Snyder, Charles D. ;Tyler, Frank H.

• Publisher: Wiley (John Wiley & Sons)
• Year: 1940
• Tongue: English
• Weight: 741 KB
• Volume: 16
• Category: Article
• ISSN: 0095-9898
• DOI: 10.1002/jcp.1030160202

✦ Synopsis

FOUR FIGURES

During experiments perfusing turtles' livers (Snyder, et al., '38) it became desirable to determine the rate of gaseous metabolism of the isolated organ under the conditions of the experiments. The following is a report of the effects on rates of the gaseous exchange under different conditions. The livers were taken from Pseudemys elegans and a box turtle, probably Terrapene Carolina.

The procedures and methods for gas-analysis used were those recommended by Peters and Van Slyke ('32), the method of Van Slyke and Neil1 ( '24, ,27) for blood plasma being followed in particular, since our liver preparations were perfused with physiological salt solutions, thus carrying in the outflow a minimum of protein. The final method adopted to prevent escape of gas from the samples was to insert stop-cock pipettes of 2 ml. capacity directly into both inflow and outflow paths. Whenever samples were wanted for analysis these pipettes, being connected by rubber tubing, could be easily removed and replaced by other clean pipettes.

By means of two-way cocks either inflowing or outflowing fluid was sent through a continuously recording glass-electrode pH-meter of Beckmann construction. I n order to record graphically the pH, a tightly fitting pulley-disk of ca. 15 em. diameter was attached to the knob operating the pH-dial indicator ; a cable connecting this pulley-disk, by way of pulley wheels, to a vertical writing point enabled one to keep a fairly constant trace-line on the moving drum, the excursions of which could be calibrated in terms of pH. The p H of inflow was noted only a t This research was supported in part by an allotment from the Rockefeller Foundation Fluid a A preliminary report of this work and partial stnteinent of the results appear in the Proc.