Oxidized low-density lipoprotein decreases the induced nitric oxide synthesis in rat mesangial cells

✍ Scribed by Zhao-Long Wu; Ming-Yu Liang; Lian-Qun Qiu

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 128 KB
• Volume: 16
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(199809)16:3<153::aid-cbf778>3.0.co;2-t

✦ Synopsis

Recently, the close relation between oxidized low density lipoprotein (Ox-LDL) and the progression of glomerular injury has been demonstrated. The nitric oxide (NO) pathway in glomerular mesangial cells may be a potential target for the adverse eects of Ox-LDL in the development of glomerular injury. In this study, we treated cultured rat mesangial cells (RMC) with Fe 2 -oxidized LDL and then stimulated the cells with lipopolysacharride (LPS, 10 mg ml 71 ). The LPS-induced NO production, assessed by NO 2 7 concentrations in cultured supernatants, decreased from 7 . 83 nmol per 10 6 cells in control to 4 . 00 nmol per 10 6 cells and 1 . 67 nmol per 10 6 cells in RMC preincubated with Ox-LDL at 20 mg ml 71 and 40 mg ml 71 , respectively (P 5 0 . 01). Native LDL had no signi®cant eects on LPS-induced NO production. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we could not detect signi®cant alteration of inducible NO synthase (iNOS) mRNA levels in RMC preincubated with Ox-LDL. Our results suggest that Ox-LDL decreases induced NO production in RMC, which may contribute to the adverse eects of Ox-LDL in progressive glomerular injury. The mechanisms of this decrease may not involve changes of iNOS genic transcription.