Bleomycin (BLM)-mediated degradation of parallel-stranded (ps) DNA duplex was investigated by HPLC product analysis of the oxidized DNA fragments. It was revealed that Co-and Fe-BLMs effectively degraded a ps DNA decamer at 5 -GC site in a similar manner to that for antiparallel-stranded (aps) B-for
Oxidative DNA strand scission by metallo bleomycins
β Scribed by Teruo Matsuura; Isao Saito; Hiroshi Sugiyama; Takaaki Sekiyama; Yukihiko Dannoue; Takashi Sera; Takashi Morii; Ryu Nagata
- Book ID
- 107901125
- Publisher
- Elsevier Science
- Year
- 1989
- Tongue
- English
- Weight
- 87 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0162-0134
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## Abstract Comet assay was applied to study induction and repair of DNA damage produced by bleomycin in barley genome. Experimental conditions were adapted to achieve efficient detection of both DNA singleβ and doubleβstrand breaks. Substantial increase of the parameter β% of DNA in tailβ was obse