✦ LIBER ✦
Metallo-bleomycin cleaves parallel-stranded DNA similarly to B-DNA
✍ Scribed by Shuji Ikeda; Isao Saito; Hiroshi Sugiyama
- Book ID
- 104210294
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- French
- Weight
- 109 KB
- Volume
- 41
- Category
- Article
- ISSN
- 0040-4039
No coin nor oath required. For personal study only.
✦ Synopsis
Bleomycin (BLM)-mediated degradation of parallel-stranded (ps) DNA duplex was investigated by HPLC product analysis of the oxidized DNA fragments. It was revealed that Co-and Fe-BLMs effectively degraded a ps DNA decamer at 5 -GC site in a similar manner to that for antiparallel-stranded (aps) B-form DNA. While the strand orientations of ps and aps duplexes are quite different, metallo-BLMs still bind to ps DNA duplex and cause DNA degradation. These results indicate that metallo-BLMs productively interact with the degradable strand of both ps and aps DNA duplexes.