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Oxidation state of the active-site cysteine in protein tyrosine phosphatase 1B

✍ Scribed by van Montfort, Rob L. M.; Congreve, Miles; Tisi, Dominic; Carr, Robin; Jhoti, Harren


Book ID
109891120
Publisher
Nature Publishing Group
Year
2003
Tongue
English
Weight
341 KB
Volume
423
Category
Article
ISSN
0028-0836

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The oxidation state of the cysteine residue at the active site of human protein tyrosine phosphatase (PTP-1B) greatly affects its enzymatic activity. We wished to examine peroxide-treated preparations for modifications of this enzyme with electrospray mass spectrometry in order to determine the loca