Oxidation of 2-deoxyglucose by human polymorphonuclear leukocytes
β Scribed by Lawrence R. Dechatelet; Thimysta L. Campbell; Charles E. McCall; Pamela S. Shirley; C. Lee Swendsen
- Publisher
- Springer
- Year
- 1980
- Tongue
- English
- Weight
- 570 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0360-3997
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## Abstract 2βDeoxyglucose transport was characterized in human polymorphonuclear leukocytes (PMN) and rabbit alveolar macrophages (AM). The Km was 1 mM for human PMN and 1.6 mM for rabbit AM, and the Vmax was 0.66 Γ 10^β3^ ΞΌmoles/45 sec/10^6^ PMN and 5.09 Γ 10^β4^ ΞΌmoles/45 sec/10^6^ AM. The rate
## Abstract The effects of 2βdeoxyglucose (DOG), an inhibitor of glycolysis, on guinea pig polymorphonuclear leukocytes (PMN) obtained from peritoneal exudates was examined. ATP levels in PMN were reduced by 40% by one hour following an incubation with 2βdeoxyglucose. When complement (C3) coated ^1
The effect of n-hexane metabolites on human polymorphonuclear leukocyte chemotaxis and luminol-dependent chemiluminescence was investigated. No effect was detected when 2-hexanol, 2-hexanone and gamma-valerolactone were used; 2,5-hexanedione at 75 micrograms/ml inhibited chemotaxis and a direct corr