Impairment of human polymorphonuclear leukocyte chemotaxis by 2,5-hexanedione

✍ Scribed by Mario Governa; Matted Valentino; Isa Visona'; Marsilia Rocco

• Publisher: Springer
• Year: 1986
• Tongue: English
• Weight: 375 KB
• Volume: 2
• Category: Article
• ISSN: 0742-2091
• DOI: 10.1007/bf00117705

✦ Synopsis

The effect of n-hexane metabolites on human polymorphonuclear leukocyte chemotaxis and luminol-dependent chemiluminescence was investigated. No effect was detected when 2-hexanol, 2-hexanone and gamma-valerolactone were used; 2,5-hexanedione at 75 micrograms/ml inhibited chemotaxis and a direct correlation between increasing the xenobiotic concentration and the degree of inhibition was found. Chemotactic peptide-induced chemiluminescence was not affected by 2,5-hexanedione. In order to clarify the phenomenon, plasma membrane fluidity was investigated by fluorescence polarization of the fluorescent probe trimethylammonium diphenylhexatriene. 2,5-hexanedione increased the membrane fluidity, while the other n-hexane metabolites did not change the degree of fluorescence polarization. Results suggest that the cellular functions modulated by membrane-cytoskeletal organization are affected by 2,5-hexanedione also at the low concentrations.