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Oxaloacetate decarboxylase ofVibrio cholerae: purification, characterization, and expression of the genes inEscherichia coli

✍ Scribed by Pius Dahinden; Yolanda Auchli; Thierry Granjon; Malgorzata Taralczak; Markus Wild; Peter Dimroth

Publisher
Springer
Year
2005
Tongue
English
Weight
392 KB
Volume
183
Category
Article
ISSN
0302-8933

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The recently cloned cDNA for pea chloroplast thioredoxin f was used to produce, by PCR, a fragment coding for a protein lacking the transit peptide. This cDNA fragment was subcloned into a pET expression vector and used to transform E. coli cells. After induction with IPTG the transformed cells prod