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Overproduction, purification and characterization of GroES and GroEL from thermophilic Bacillus stearothermophilus

✍ Scribed by Ulrike Schön; Wolfgang Schumann


Book ID
109320892
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
612 KB
Volume
134
Category
Article
ISSN
0378-1097

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Purification and characterization of the
✍ John O. Chikwem; Ronald J. Downey 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 546 KB

The reduced methylviolegen-nitrate reductase of the obligate thermophile, Bacillus stearothermophilus NCA strain 2 I84 (ATCC 120 16), has been purified to electrophoretic homogeneity, 53-fold with a yield of 12.5%. The purification procedure involved solubilization with octyl glucoside, ammonium sul