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Overproduction, isolation and determination of the amino-terminal sequence of the SecY protein, a membrane protein involved in protein export in Escherichia coli

✍ Scribed by Yoshinori AKIYAMA; Koreaki ITO


Book ID
115124664
Publisher
John Wiley and Sons
Year
1986
Tongue
English
Weight
919 KB
Volume
159
Category
Article
ISSN
1432-1327

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πŸ“œ SIMILAR VOLUMES


Identification of the secY (prlA) gene p
✍ Ito, Koreaki πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 586 KB

The gene secY (or prlA) is essential for protein export across the cytoplasmic membrane of Escherichia coli. The protein product of secY has been identified using the gene cloned under the control of the pL promoter of phage lambda in combination with the maxicell system. The protein was found to ha

The use of extragenic suppressors to def
✍ Brickman, Edith R. ;Oliver, Donald B. ;Garwin, Jeffrey L. ;Kumamoto, Carol ;Beck πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 469 KB

The secA gene codes for a membrane component involved in protein export in E. coli. In order to define other genes whose products play such a role, we have characterized extragenic suppressors of a secA(Ts) mutation. These suppressors fall into at least three genetic loci. One such locus is the prlA