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The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

✍ Scribed by Brickman, Edith R. ;Oliver, Donald B. ;Garwin, Jeffrey L. ;Kumamoto, Carol ;Beckwith, Jon

Publisher: Springer
Year: 1984
Tongue: English
Weight: 469 KB
Volume: 196
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334087

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✦ Synopsis

The secA gene codes for a membrane component involved in protein export in E. coli. In order to define other genes whose products play such a role, we have characterized extragenic suppressors of a secA(Ts) mutation. These suppressors fall into at least three genetic loci. One such locus is the prlA gene, previously identified by mutations which suppress signal sequence mutants. Thus, this approach may allow the identification of new genes involved in the export process.

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