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Overproduction and Purification of the Colicin E1 Immunity Protein

✍ Scribed by Komei Shirabe; Mamoru Yamada; Allan R. Merrill; William A. Cramer; Atsushi Nakazawa


Book ID
112263122
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
220 KB
Volume
29
Category
Article
ISSN
0147-619X

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Effect of base substitutions in the coli
✍ Yamada, Mamoru ;Nakazawa, Atsushi πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 987 KB

Base substitutions have been introduced into the segment of the colicin E1 gene corresponding to the polypeptide region between the 404th and the 502nd residues which was considered to participate in colicin E1 export and bacteriocin activity. The methods used were in vitro localized mutagenesis wit