𝔖 Bobbio Scriptorium
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Cloning and overexpression of the colicin E1 immunity gene

✍ Scribed by Armen B. Shanafelt; Kenneth M. Goldman; Robert A. Kastelein; Celik Kayalar


Book ID
119078922
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
401 KB
Volume
17
Category
Article
ISSN
0147-619X

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Effect of base substitutions in the coli
✍ Yamada, Mamoru ;Nakazawa, Atsushi πŸ“‚ Article πŸ“… 1986 πŸ› Springer 🌐 English βš– 987 KB

Base substitutions have been introduced into the segment of the colicin E1 gene corresponding to the polypeptide region between the 404th and the 502nd residues which was considered to participate in colicin E1 export and bacteriocin activity. The methods used were in vitro localized mutagenesis wit

Cloning and expression of the activity a
✍ Γ–lschlΓ€ger, Tobias ;Schramm, Edgar ;Braun, Volkmar πŸ“‚ Article πŸ“… 1984 πŸ› Springer 🌐 English βš– 651 KB

The activity and immunity genes for colicins B and M on two conjugative ColBM plasmids, pCl139 and pF166, were cloned into pBR322 and pACYC184, respectively. The colicin regions on both recombinant plasmids were identical with regard to restriction endonuclease sites and the arrangement of the genes