Cloning and overexpression of the colicin E1 immunity gene
β Scribed by Armen B. Shanafelt; Kenneth M. Goldman; Robert A. Kastelein; Celik Kayalar
- Book ID
- 119078922
- Publisher
- Elsevier Science
- Year
- 1987
- Tongue
- English
- Weight
- 401 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0147-619X
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Base substitutions have been introduced into the segment of the colicin E1 gene corresponding to the polypeptide region between the 404th and the 502nd residues which was considered to participate in colicin E1 export and bacteriocin activity. The methods used were in vitro localized mutagenesis wit
The activity and immunity genes for colicins B and M on two conjugative ColBM plasmids, pCl139 and pF166, were cloned into pBR322 and pACYC184, respectively. The colicin regions on both recombinant plasmids were identical with regard to restriction endonuclease sites and the arrangement of the genes