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Overproduction and large-scale preparation of deoxyuridine triphosphate nucleotidohydrolase from Escherichia coli

✍ Scribed by Ingrid HOFFMANN; John WIDSTRÖM; Michael ZEPPEZAUER; Per Olof NYMAN


Book ID
115125010
Publisher
John Wiley and Sons
Year
1987
Tongue
English
Weight
731 KB
Volume
164
Category
Article
ISSN
1432-1327

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✍ Dr. Eilia S. Cedergren-Zeppezauer; Gunilla Larsson; Ingrid Hoffmann; Karl W. Tör 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 563 KB

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase), an enzyme in the nucleotide metabolism that is a pyrophosphatase hydrolyzing dUTP, has been crystallized. The crystals belong to the trigonal space group R3 and diffract beyond 2 A. The native dUTPase crystals and a mercury derivative are stab

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✍ Beata G. Vertessy 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 233 KB

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase), a ubiquitous enzyme of DNA metabolism, has been implicated as a novel target of anticancer and antiviral drug design. This task is most efficiently accomplished by X-ray crystallography of the relevant protein-inhibitor complexes. However, the