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Overexpression of the Wilms' tumor gene WT1 in de novo lung cancers

โœ Scribed by Yusuke Oji; Shinichiro Miyoshi; Hajime Maeda; Seiji Hayashi; Hiroya Tamaki; Shin-Ichi Nakatsuka; Masayuki Yao; Eigo Takahashi; Yoko Nakano; Hirohisa Hirabayashi; Yasushi Shintani; Yoshihiro Oka; Akihiro Tsuboi; Naoki Hosen; Momotaro Asada; Tatsuya Fujioka; Masaki Murakami; Keisuke Kanato; Mari Motomura; Eui Ho Kim; Manabu Kawakami; Kazuhiro Ikegame; Hiroyasu Ogawa; Katsuyuki Aozasa; Ichiro Kawase; Haruo Sugiyama

Publisher
John Wiley and Sons
Year
2002
Tongue
French
Weight
627 KB
Volume
100
Category
Article
ISSN
0020-7136

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โœฆ Synopsis

Abstract

Expression of the Wilms' tumor gene WT1 in de novo lung cancer was examined using quantitative realโ€time RTโ€PCR and immunohistochemistry. Overexpression of the WT1 gene was detected by RTโ€PCR in 54/56 (96%) de novo nonโ€small cell lung cancers examined and confirmed by detection of WT1 protein with an antiโ€WT1 antibody. Overexpression of the WT1 gene was also demonstrated in 5/6 (83%) de novo small cell lung cancers by immunohistochemistry. Furthermore, when the WT1 gene was examined for mutations by direct sequencing of genomic DNA in 7 lung cancers, no mutations were found. These results suggest that the nonmutated, wildโ€type WT1 gene plays an important role in tumorigenesis of de novo lung cancers and may provide us with the rationale for new therapeutic strategies for lung cancer targeting the WT1 gene and its products. ยฉ 2002 Wileyโ€Liss, Inc.

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