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Overexpression of the Bacillus subtilis and circulans Xylanases in Escherichia coli

✍ Scribed by W.L. Sung; C.K. Luk; D.M. Zahab; W. Wakarchuk

Book ID
115644744
Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
542 KB
Volume
4
Category
Article
ISSN
1046-5928

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We have cloned a 14 kb DNA segment containing the coding sequence (polC) for DNA polymerase III (PolIII) from the Bacillus subtilis chromosome. The plasmid carrying the sequence, pRO10, directs the synthesis of the 160 kDa PolIII protein and three additional polypeptides in Escherichia coli maxicell