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Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli

โœ Scribed by Bernier, R.; Driguez, H.; Desrochers, M.


Book ID
122697448
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
987 KB
Volume
26
Category
Article
ISSN
0378-1119

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A composite plasmid (pAT2010) has been constructed in vitro from RSF2124 and Bacillus subtilis IFO3022 plasmid (pAT1060) by covalent joining of the two DNA molecules by means of Escherichia coli DNA ligase through the cohesive ends generated by restriction endonuclease RI (EcoRI) cleavage. The compo