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Overexpression of protein disulfide isomerases enhances secretion of recombinant human transferrin inSchizosaccharomyces pombe

✍ Scribed by Hiroyuki Mukaiyama; Hideki Tohda; Kaoru Takegawa

Book ID: 105952457
Publisher: Springer
Year: 2009
Tongue: English
Weight: 354 KB
Volume: 86
Category: Article
ISSN: 1432-0614
DOI: 10.1007/s00253-009-2393-x

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Overexpression of recombinant proteins in animal cells is commonly achieved by using gene amplification techniques. Gene amplified cells possess up to several thousand genes coding for the target protein. Constitutive expression of these genes leads to high levels of the corresponding mRNA species