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Overexpression of a small medicinal peptide from ginseng in the yeast Pichia pastoris

โœ Scribed by Yajun Yan; Jingchun Chen; Jun Li

Book ID
117702719
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
192 KB
Volume
29
Category
Article
ISSN
1046-5928

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Human insulin from a precursor overexpre
Human insulin from a precursor overexpressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product
โœ Yan Wang; Zhen-He Liang; You-Shang Zhang; Shi-Yin Yao; Ying-Gao Xu; Yue-Hua Tang ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 281 KB

The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor. A transformant with a high copy number of the gene integrated into the chromosome was obtained by the dot-blotting method. In high-density fermentation