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Human Proinsulin C-peptide from a Precursor Overexpressed in Pichia pastoris

โœ Scribed by Yang-Bin HUANG; Jiang LI; Xin GAO; Jiu-Ru SUN; Yi LU; Tao FENG; Jian FEI; Da-Fu CUI; Qi-Chang XIA; Jun REN; You-Shang ZHANG

Book ID
111357703
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
639 KB
Volume
38
Category
Article
ISSN
1672-9145

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๐Ÿ“œ SIMILAR VOLUMES

Human insulin from a precursor overexpre
Human insulin from a precursor overexpressed in the methylotrophic yeast Pichia pastoris and a simple procedure for purifying the expression product
โœ Yan Wang; Zhen-He Liang; You-Shang Zhang; Shi-Yin Yao; Ying-Gao Xu; Yue-Hua Tang ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 281 KB

The methylotrophic yeast Pichia pastoris, which proved successful in producing many heterologous proteins, was used to express an insulin precursor. A transformant with a high copy number of the gene integrated into the chromosome was obtained by the dot-blotting method. In high-density fermentation