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Over-expression of α-lytic protease and its mutants by recombinantEscherichia coli

✍ Scribed by Kevin D. Haggett; Lloyd D. Graham; Denis S. Wright; Robert G. Whittaker


Publisher
Springer Netherlands
Year
1994
Tongue
English
Weight
432 KB
Volume
16
Category
Article
ISSN
0141-5492

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Rapid purification of α-lytic protease a
✍ Kevin D. Haggett; Lloyd D. Graham; Robert G. Whittaker 📂 Article 📅 1994 🏛 Springer-Verlag 🌐 English ⚖ 724 KB

A single-step procedure is small scale (annrox. 5mg) described for purifying a-lytic protease and its mutants on a from cell-free supematants of recombinant E. coli. Although free of non-enzyme pro&t, these preparations often contain fragments that arise from enzymatic self-digestion. Two strong cat