Rapid purification of α-lytic protease a
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Kevin D. Haggett; Lloyd D. Graham; Robert G. Whittaker
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Article
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1994
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Springer-Verlag
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English
⚖ 724 KB
A single-step procedure is small scale (annrox. 5mg) described for purifying a-lytic protease and its mutants on a from cell-free supematants of recombinant E. coli. Although free of non-enzyme pro&t, these preparations often contain fragments that arise from enzymatic self-digestion. Two strong cat