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Over-expression of proteins using a modified pBAD24 vector inE. coliexpression system

✍ Scribed by Sampali Banerjee; Shardul S. Salunkhe; Anjali D. Apte-Deshpande; Naganath S. Mandi; Goutam Mandal; Sriram Padmanabhan


Book ID
106364677
Publisher
Springer Netherlands
Year
2009
Tongue
English
Weight
257 KB
Volume
31
Category
Article
ISSN
0141-5492

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We report the effects of a strong overexpression of the GAL4 activator protein on the expression of UASGAL regulated genes, obtained by cloning the GAL4 gene and the GALl-10 upstream activating sequence (UAS~AL)-lacZ fusion in the same high copy number plasmid. Comparable amounts of active enzyme we