The folding of randomly coiled poly(bg1utamic acid) to the helical state has been studied in N-methylacetamide by titration methods. Since this solvent would be expected to form amide-peptide group hydrogen bonds with the unfolded form of the polymer, to a first approximation no helix stabilization
Osmotically Induced Helix-Coil Transition in Poly(Glutamic Acid)
✍ Scribed by Stanley, Christopher B.; Strey, Helmut H.
- Book ID
- 119921653
- Publisher
- Biophysical Society
- Year
- 2008
- Tongue
- English
- Weight
- 342 KB
- Volume
- 94
- Category
- Article
- ISSN
- 0006-3495
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## Abstract A polarimetric electric‐field‐jump relaxation apparatus is described and used to determine the relaxation spectrum for the helix–coil transition of poly(α,L‐glutamic acid) in water at 24°C. A maximum relaxation time of 1.7 μc occurs at the transition midpoint (pH = 5.9) yielding a rate
Potentiometric titrations and some complementary optical rotation data are presented for solutions of poly(L-glntamic acid) (PGA) in several H,O-ethanol mixtnres. The data allow the determination of the intrinsic p K (pKo), slope of the apparent pK (pKaPp), versus degree of ionization curves and of