Osmotic Response of Large Unilamellar Vesicles of Phosphatidylcholine: Factors Determining the Rate of the Process and the Properties of the Shrunken Vesicles
✍ Scribed by E.B. Abuin; A.M. Campos; E.A. Lissi; E.A. Disalvo
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 321 KB
- Volume
- 171
- Category
- Article
- ISSN
- 0021-9797
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Phosphatidylcholine (PC)-cholesterol (0 -30 mol%) unilamellar vesicles of several sizes (20 -600 nm) were prepared in buffer (pH 7.4) solutions by sonication or extrusion methods. The vesicle size was measured by a dynamic light-scattering method. Absorption spectra of chlorpromazine (CPZ) and trifl
Vesicles having diameters from 20 to 200 nm were prepared from egg-yolk phosphatidylcholine (PC) and were separated as well as analyzed by methods that can be carried out with standard laboratory equipment. Gel-chromatography on Sephacryl S 1000 was adapted for expeditious size analysis of vesicles
## Abstract Electrofusion of giant unilamellar vesicles (GUVs) and living cells is a gentle method for delivering membrane impermeable cryo‐/lyo‐protective molecules into living cells for cryo‐/lyopreservation. In this method, an optimal length and strength of an electric pulse are required to init