Orthophosphate determination: A simple spectrophotometric method for the measurement of the orthophosphate-triethylamine-molybdate complex
β Scribed by Lagunas, Rosario ;DelaFuente, Gertrudis
- Book ID
- 105113880
- Publisher
- Springer-Verlag
- Year
- 1979
- Weight
- 192 KB
- Volume
- 72
- Category
- Article
- ISSN
- 0344-838X
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We describe a modified colorimetric method that quantitates inorganic phosphate linearly up to 60 nmol, with high stability of the developed color and with a low interference by ATP concentration (up to 30 mM). This method is very suitable for use in ATPase enzymatic assays, especially with enzymes
We report below on an extremely simple and somewhat more sensitive modification of the Chert procedure (1) for inorganic orthophosphate determination that has been routinely used in this laboratory for almost 2 years. The method overcomes the problem of precipitation of the phosphomolybdate complex