Origin of multiple bands of proteins on sodium dodecyl sulphate-polyacrylamide gel electrophoresis - intermolecular disulphide cross-linking due to the presence of oxidizing components in the reducing agent

Multiple Bands on the Sodium Dodecyl Sul

Multiple Bands on the Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Gels of Proteins Due to Intermolecular Disulfide Cross-Linking

✍ T.K.S. Kumar; K. Gopalakrishna; V.V.H. Prasad; M.W. Pandit 📂 Article 📅 1993 🏛 Elsevier Science 🌐 English ⚖ 289 KB

Gel electrophoresis has been used extensively as an analytical technique to check the purity and to determine the molecular weight of proteins. Improved levels of detection of proteins by silver staining of the gels have made the technique more sensitive in detecting heterogeneity. We report here so