## Synopsis Sonicated calf-thymus DNA (200 i 30 base pairs) spontaneously forms viscoelastic gels over a wide range of concentration, temperature, and buffer conditions. Quasielastic light scattering (QLS) can be used to monitor this process, because the ratio of dynamicto-static scattering intens
Orientational interactions in low-concentration DNA solutions
β Scribed by Christian Marion; Bernard Roux; Maxime Hanss
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1983
- Tongue
- English
- Weight
- 680 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
The rotational relaxation time 73 of DNA molecules (M, N 5 X lo6) in solution has been determined by the transient electric birefringence method. The analysis of the birefringence decay makes it possible to study only the higher-molecular-weight fraction, the molecules being considered as rigid elongated particles in a short time scale. A marked concentration dependence of the relaxation time has been observed for DNA in low ionic strengths. Above a critical concentration c*, 7 3 increases with the DNA concentration, c . The value of c* increases with the ionic strength. For ionic strength (with NaCl), c* is about 10 pg/mL; then we observe the same strong concentration dependence of rotational relaxation times as recently reported for rodlike M-13 viruses [Maguire,
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## Abstract Proteinβprotein interactions were measured for ovalbumin and for lysozyme in aqueous salt solutions. Proteinβprotein interactions are correlated with a proposed potential of mean force equal to the free energy to desolvate the protein surface that is made inaccessible to the solvent due