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Orientational interactions in low-concentration DNA solutions

✍ Scribed by Christian Marion; Bernard Roux; Maxime Hanss


Publisher
Wiley (John Wiley & Sons)
Year
1983
Tongue
English
Weight
680 KB
Volume
22
Category
Article
ISSN
0006-3525

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✦ Synopsis


The rotational relaxation time 73 of DNA molecules (M, N 5 X lo6) in solution has been determined by the transient electric birefringence method. The analysis of the birefringence decay makes it possible to study only the higher-molecular-weight fraction, the molecules being considered as rigid elongated particles in a short time scale. A marked concentration dependence of the relaxation time has been observed for DNA in low ionic strengths. Above a critical concentration c*, 7 3 increases with the DNA concentration, c . The value of c* increases with the ionic strength. For ionic strength (with NaCl), c* is about 10 pg/mL; then we observe the same strong concentration dependence of rotational relaxation times as recently reported for rodlike M-13 viruses [Maguire,


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