Orientation and capturing of antibody affinity ligands: Applications to surface plasmon resonance biochips

✍ Scribed by Gunnar Bergström; Carl-Fredrik Mandenius

• Publisher: Elsevier Science
• Year: 2011
• Tongue: English
• Weight: 599 KB
• Volume: 158
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/j.snb.2011.06.017

✦ Synopsis

A surface plasmon resonance (SPR) sensor chip with immobilized protein G was used for simultaneously capturing, purifying and orienting antibody ligands. The ligands were further stabilized by chemical cross-linking. This procedure of designing the sensor chip improved efficient use of the ligands and could prolong the analytical use.

The procedure was evaluated on standard dextran-coated sensor chips onto which commercial semipurified antibodies towards human serum albumin and human troponin where captured and used for analysing their antigens.

The procedure demonstrates a general design approach for presenting the biorecognition element on a biosensor surface which enhances sensitivity, stability and selectivity at the same time as an impure ligand is purified.