Organometallic complexes with biological molecules: V. In vivo cytotoxicity of diorganotin(IV)-amoxicillin derivatives in mitotic chromosomes of rutilus rubilio (pisces, cyprinidae)

✍ Scribed by Roberto Vitturi; Bruno Zava; Maria Stella Colomba; Alessandro Pellerito; Francesco Maggio; Lorenzo Pellerito

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 527 KB
• Volume: 9
• Category: Article
• ISSN: 0268-2605
• DOI: 10.1002/aoc.590090710

✦ Synopsis

In order to test in uiuo cytotoxicity of diorganotin(1V)-amoxicillin (amox) derivatives, mitotic chromosomes of Rutilus rubilio (Pisces, Cyprinidae) have been analyzed using two different chromosome-staining techniques.

Results gathered after exposure of fish to the free amox. 3H,O, R,SnClamox. 2H,O, and R,Snamox, -2H20 (R =methyl, butyl and phenyl; amox-= 6-[~(-)-~-amino-p-hydroxyphenylacetamido]penicillinate) suggest that methyl derivatives seem to exert a lower cytotoxicity than butyl and phenyl ones and that R,Snamox, .2H,O derivatives are more toxic than R,SnClamox * 2H,O at both and mol dm-j concentrations. The following structural lesions have been identified by comparative analysis of mitotic chromosomes from untreated specimens (controls) and specimens treated with diorganotin(1V)amoxicillin derivatives: (1) differentially stained chromosome areas; (2) granular deeply stained zones along the chromosomal body; (3) arm breakages; and (4) side-arm bridges (pseudochiasmata).