𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Organic solvent tolerance of Halobacterium sp. SP1(1) and its extracellular protease

✍ Scribed by Aparna V. Akolkar; Gauravi M. Deshpande; Kandarp N. Raval; Deepa Durai; Anuradha S. Nerurkar; Anjana J. Desai

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
88 KB
Volume
48
Category
Article
ISSN
0233-111X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Halophilic archaea belonging to three different genera‐ Halobacterium, Haloarcula and Haloferax, were isolated from Kandla salt pans. The isolates had an optimum requirement of 25% NaCl for growth. Increase in organic solvent tolerance of isolates was observed at higher NaCl concentrations. Among the three isolates Halobacterium sp. SP1(1) was found to be more tolerant than Haloarcula sp. SP2(2) and Haloferax sp. SP1(2a). The extracellular protease of Halobacterium sp. SP1(1) showed higher solvent tolerance compared to the organism itself. The enzyme was highly tolerant to toluene, xylene, n‐decane, n‐dodecane and n‐undecane, majority of which are frequently used in paints. These findings may help in understanding the mechanism of organic solvent tolerance in halophilic archaea and their application in antifouling coatings. Also, best to our knowledge the present study is the first report on organic solvent tolerance of haloarchaeal extracellular protease. (Β© 2008 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

πŸ“œ SIMILAR VOLUMES

Isolation and screening of an extracellu
Isolation and screening of an extracellular organic solvent-tolerant protease producer
✍ Lee Poh Geok; Che Nyonya Abdul Razak; Raja Noor Zaliha Abd Rahman; Mahiran Basri πŸ“‚ Article πŸ“… 2003 πŸ› Elsevier Science 🌐 English βš– 193 KB

An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xyleneethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified

Purification and characterization of an
Purification and characterization of an organic solvent and detergent-tolerant novel protease produced by Bacillus sp. RKY3
✍ LVA Reddy; Young-Jung Wee; Hwa-Won Ryu πŸ“‚ Article πŸ“… 2008 πŸ› Wiley (John Wiley & Sons) 🌐 English βš– 161 KB

## Abstract **BACKGROUND:** Purification and characterization of a novel protease produced by __Bacillus__ sp. RKY3, has been investigated, with special emphasis on the stability of the enzyme in the presence of different oxidizing and reducing agents as well as organic solvents. The enzyme was pur

Constitutive expression and secretion of
Constitutive expression and secretion of proteases in non-metastatic SP1 mammary carcinoma cells and its metastatic sublines
✍ Bozena Korczak; Robert S. Kerbel; James Dennis πŸ“‚ Article πŸ“… 1991 πŸ› John Wiley and Sons 🌐 French βš– 731 KB

## Abstract Malignant tumors are generally characterized by extensive local tissue invasion and destruction of ECM which may be due to increased constitutive expression and activity of secreted proteases. Moreover, a large number of diverse protease activities may be constitutively over‐expressed i