✦ LIBER ✦

Isolation and screening of an extracellular organic solvent-tolerant protease producer

✍ Scribed by Lee Poh Geok; Che Nyonya Abdul Razak; Raja Noor Zaliha Abd Rahman; Mahiran Basri; Abu Bakar Salleh

Publisher: Elsevier Science
Year: 2003
Tongue: English
Weight: 193 KB
Volume: 13
Category: Article
ISSN: 1369-703X
DOI: 10.1016/s1369-703x(02)00137-7

No coin nor oath required. For personal study only.

✦ Synopsis

An extracellular organic solvent-tolerant protease producer has been successfully isolated out of 11 isolates of benzene-toluene-xyleneethylbenzene (BTEX) tolerant bacteria. This organic solvent-tolerant microorganism was found to be a polycyclic-aromatic-hydrocarbons (PAHs) degrader and identified as Pseudomonas aeruginosa strain K. It was selected based on the stability of its proteolytic enzyme in the presence of various organic solvents. P. aeruginosa strain K protease tolerated up to at least 50% (v/v) of benzene, n-hexane, 1-decanol, isooctane and n-hexadecane and was also stable in the presence of 25% (v/v) n-decane and n-dodecane. This enzyme strain K was activated 2.5, 1.5 and 1.2 times by 75% (v/v) of 1-decanol, isooctane and n-dodecane, respectively. This organic solvent stable protease could be used as a biocatalyst for enzymatic synthesis in the presence of organic solvents.

📜 SIMILAR VOLUMES

Isolation and screening of a novel extra

Isolation and screening of a novel extracellular organic solvent-stable protease producer

✍ Yaowei Fang; Shu Liu; Shujun Wang; Mingsheng Lv 📂 Article 📅 2009 🏛 Elsevier Science 🌐 English ⚖ 223 KB

Organic solvent tolerance of Halobacteri

Organic solvent tolerance of Halobacterium sp. SP1(1) and its extracellular protease

✍ Aparna V. Akolkar; Gauravi M. Deshpande; Kandarp N. Raval; Deepa Durai; Anuradha 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 88 KB

## Abstract Halophilic archaea belonging to three different genera‐ __Halobacterium__, __Haloarcula__ and __Haloferax__, were isolated from Kandla salt pans. The isolates had an optimum requirement of 25% NaCl for growth. Increase in organic solvent tolerance of isolates was observed at higher NaCl

Purification and characterization of an

Purification and characterization of an organic solvent and detergent-tolerant novel protease produced by Bacillus sp. RKY3

✍ LVA Reddy; Young-Jung Wee; Hwa-Won Ryu 📂 Article 📅 2008 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 161 KB

## Abstract **BACKGROUND:** Purification and characterization of a novel protease produced by __Bacillus__ sp. RKY3, has been investigated, with special emphasis on the stability of the enzyme in the presence of different oxidizing and reducing agents as well as organic solvents. The enzyme was pur

Biochemical and physiological properties

Biochemical and physiological properties of an extracellular protease produced by Bacillus megateriurn

✍ J. Millet; R. Acher; J.-P. Aubert 📂 Article 📅 1969 🏛 John Wiley and Sons 🌐 English ⚖ 628 KB

Molecular investigation of a gene encodi

Molecular investigation of a gene encoding organic solvent-tolerant alkaline protease from Pseudomonas aeruginosa strain K

✍ Raja Noor Zaliha Raja Abd. Rahman; Lee Poh Geok; Chee Fah Wong; Mahiran Basri; A 📂 Article 📅 2010 🏛 John Wiley and Sons 🌐 English ⚖ 207 KB

## Abstract A gene encoding an organic solvent‐stable protease was amplified from __Pseudomonas aeruginosa__ strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from __Pseudomonas__ species. The gene, which consist

FR901512, a Novel HMG-CoA Reductase Inhi

FR901512, a Novel HMG-CoA Reductase Inhibitor Produced by an Agonomycetous Fungus No. 14919. Part 1. Taxonomy of the Producing Organism, Fermentation, Isolation and Physico-chemical Properties.

✍ Hidetaka Hatori; Bunji Sato; Ikuko Sato; Toshihiro Shibata; Yasuhisa Tsurumi; Sa 📂 Article 📅 2004 🏛 John Wiley and Sons ⚖ 58 KB 👁 1 views