Organic solvent and pH induced alteration of product specificity of CGTase
β Scribed by Kyo-Sun Park; Hyun-Mi Oh; Hui-Woog Choe; Chung-Ung Park; Kang-Min Lee
- Publisher
- The Korean Society for Biotechnology and Bioengineering
- Year
- 1998
- Tongue
- English
- Weight
- 555 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1226-8372
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## Abstract Electrochromatography is demonstrated using a 50 ΞΌm i.d. capillary column. The separation of solutes due to their different mobilities is obtained by the application of high voltage along the column. The elution times of solutes are dependent on the pH and composition of the eluent. Bot
A simple methodology has been successfully employed to explain the solvent dependence of the substrate specificity of enzymes in organic media. This methodology, which does not require the knowledge of the enzyme structure and is thus applicable to lyophilized and other noncrystalline enzyme prepara
Partitioning of a variety of nonionic and acidic organic compounds was examined in aqueous dextranΒ±poly(ethylene glycol) (PEG) two-phase systems of different ionic composition at pH 2.00 and 7.40. Relative hydrophobicity of the compounds was estimated in terms of equivalent numbers of methylene unit