✦ LIBER ✦

Effects of pH and organic solvent on chromatographic behavior in capillary electrochromatography

✍ Scribed by Shinya Kitagawa; Takao Tsuda

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 381 KB
Volume: 6
Category: Article
ISSN: 1040-7685
DOI: 10.1002/mcs.1220060202

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✦ Synopsis

Abstract

Electrochromatography is demonstrated using a 50 μm i.d. capillary column. The separation of solutes due to their different mobilities is obtained by the application of high voltage along the column. The elution times of solutes are dependent on the pH and composition of the eluent. Both electrophoretic and electroosmotic flow velocities are almost constant between 30 and 90% methanol in the eluent, and they increase with more than 90% methanol. The pH dependence of the electroosmotic flow velocity may be related to the dissociation of unreacted silanol groups on the surface of the ODS‐silica particles.

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