Organ-specific expression of hepatitis B surface antigen in potato
β Scribed by N. Domansky; P. Ehsani; A -H. Salmanian; T. Medvedeva
- Publisher
- Springer Netherlands
- Year
- 1995
- Tongue
- English
- Weight
- 173 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-5492
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β¦ Synopsis
The gene encoding the hepatitis B surface antigen (HBsAg) under the control of cauliflower mosaic virus 35S-promoter was constructed and expressed in transgenic potato plants. The HBsAg expression were measured by ELISA kit containing monoclonal antibodies. The amount of HBsAg in roots was found 5-10 fold higher than in leaf tissues
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The human hepatoblastoma-derived cell line HB6 I I secretes hepatitis-B surface antigen (HBsAg) and hepatitis-B e antigen (HBeAg) into the medium. Hepatitis-B-virus(HBV) DNA integrated into the cellular genome was found to be hypermethylated. When the cells were treated with 5-azacytidine for 3 days
## Abstract Small hepatitis B surface antigen (HBsAg) is considered to be the best marker for the diagnosis of Hepatitis B virus infection. However, HBsAg variants with mutations within the βaβ determinant may be poorly or not detected by diagnostic assays. Three antiβHBsAg monoclonal antibodies (6